Multi-Probe Micromanipulator System
Multiple silicon probes. One simple micromanipulator system.
Maximize productivity and precision with automated probe positioning for acute in-vivo recording
Supports neural probes from imec Neuropixels, NeuroNexus, Cambridge Neurotech and more
The MPM Multi-Probe Micromanipulator System provides convenient, automated positioning of multiple neural probes for acute in-vivo recording in electrophysiology and optogenetic research. Simple to set up and operate, it is the first micro-manipulator designed specifically for use with silicon neural probes.
The compact hardware design enables independent positioning of multiple probes in the smallest space, with ample clear area for a virtual task environment. Automatically and independently move each probe to the optimum location in the brain.
Pathfinder Software with virtual coordinate system (VCS) enables simulation, planning and execution with all probes moving in a common stereotactic coordinate space.
NEW integration with trajectory planning and data acquisition apps promises even greater electrode placement ease and accuracy, for more scientifically valuable recordings. Watch the preview video.
Compact, easy-to-use system
Multiple probes in the smallest space
A mounting ring allows multiple probe arm assemblies to be positioned around the target – from one probe to eight or more.
Simple manual pre-adjustment
Each probe arm has four degrees of freedom for initial pre-adjustment. Use the displacement markings to register the location of each probe in stereotactic coordinates and calculate the approach angle. Enter this information into the Pathfinder Software Virtual Coordinate System (VCS) and remove real-time geometry puzzles from the time it takes to do a recording. Use the simulation mode for a 3D visualization of probe assemblies, along with target bregma and lambda locations, to plan probe positions or practice moving.
Precise motorized positioning
Each probe arm has three axes of motorized motion for automated positioning. The motorized positioners allow precise control of x-y location and z insertion depth, automatically and independently moving each probe to the optimum location in the brain. The standard motorized positioners offer 15 mm of travel on each axis, with high force and stiffness. The small, stiff, precise mechanical stages, along with an exceedingly stable mechanical loop from the probe tip to the manipulator, ensure a drift-free manipulator environment.
PATHFINDER SOFTWARE – Planning, simulation, execution
Image courtesy Rumbaugh Lab – Scripps Research
MPM Pathfinder Software with Virtual Coordinate System (VCS) simplifies planning, simulation and execution of probe insertion trajectories. Enter the start and stop coordinates to register probe locations to a 2D image or a 3D reference space using stereotatic coordinates.
The software automatically calculates all approach angles. Each probe moves independently within this global space, rather than along its local axes. This makes it much easier to position and keep track of multiple probe locations.
Use the simulation mode to plan and visualize probe positions and approach angles, plan insertion paths, and check for potential probe interference. You spend less time calculating angles and more time recording.
- Control multiple probes in stereotactic coordinates with simplicity and reproducibility.
- Simulation mode simplifies setup and training
- Control up to eight probes in a single PC window. Open additional windows for more probes.
- Coarse, fine and insertion step modes
- Automated z-axis insertion – very slow with x/y movement disabled
- Multiple editable configurations – step size, speed, axis associations, etc.
- Optional joystick or other input device control (PC compatible USB-HID)
“[VCS] takes the hassle out of multi-probe alignment… makes it much easier to keep track of probe locations when many are in use simultaneously.”
– Josh Siegle, The Allen Institute for Brain Science
Designed for High-Density Silicon Neural Probes
Neuropixels – NeuroNexus – Cambridge Neurotech
The MPM System is the first micromanipulator specifically designed for silicon neural probes. Probe mounting kits are available for Neuropixels 2.0 probes probes (alpha phase), Neuropixels 1.0 probes with silicon or metal cap, and other commercial probes. We also offer custom probe mount design.
The MPM System can be used to simultaneously position optical waveguides or optical fibers, for research combining electrophysiology with optogenetic stimulation.
Protect your probes
Our probe mounts, probe mounting kits, and alignment fixtures make it easier to attach delicate silicon probes to the manipulators. A steel reference probe reduces setup time and minimizes risk of damage to probes during setup. Once you have determined your probe angles and insertion paths using the reference probe and Pathfinder Software, repeatable stops on the probe arms allow you to simply swing the arm up to replace the reference probe with a silicon probe, and return it to the target position.
“Using the VCS system… saved a lot of time during experiments. I was really happy with the accuracy and reproducibility.”
-Tobi Leva, MDC-Berlin
MPM System Advantages
- Compact size: Independently position multiple probes in the smallest space
- Fast set-up: Fully engineered, modular apparatus space-optimized for upright or inverted experiments
- Software for simulation, planning and execution: Pathfinder Software with Virtual Coordinate System (VCS) delivers 3D visualization for approach angle planning, fast setup, and precise execution of probe insertion
- Easy operation: 24 axes of automated motion are controlled in a single PC window (open additional windows for more probes)
- Automated sub-micrometer probe adjustments with absolute position measurement and control
- Gross manual + fine automated control in one system
- Four-axis manual pre-positioning with repeatable stops
- Three-axis motorized fine positioning with 15 mm travel on each axis
- Exceedingly stable mechanical loop from probe tip to manipulator for drift-free operation
Configurable to your experiment
MPM Systems are fully configurable for inverted or upright position, number of probe arms, and type of probe holders.
In the inverted configuration, the arms are mounted to the underside of an elevated platform, maximizing clear space below the probes for animal interaction with a virtual task environment. In the upright configuration, the arms are mounted to a standard laboratory table.
Each system is complete and easy to assemble with the tools and hardware provided. All cables and connectors are labeled, including standard USB cables to the PC. The MPM software installs on your Windows PC in minutes.
NEUROSCIENCE 2022 SNEAK PREVIEW The integration of trajectory planning and data acquisition apps with the MPM Multi-Probe Micromanipulator System's Pathfinder Software will allow you to simulate, plan, execute, debug and repeat your experiments with greater ease and...
Image credit: Laser Focus World / Travis BabolaA recent article in Laser Focus World explains the basics of optogenetics and describes newer techniques, like single-cell and holographic optogenetics, that allow neuroscientists to give every cell its own code and to...
Obtaining successful in-vivo electrophysiological recordings with silicon neural probes requires a set of carefully executed activities which include: Careful planning of the optimum insertion path. Accurate placement of the probe to enter the brain at the designated...
Download software and user guides for your MPM System. Watch How-To videos on system setup and operation. (User account creation required)
Download drawings and hardware guides to plan your purchase and installation of an MPM System. (Register here if you don’t have a user account)
Neuropixels 2.0 probe (alpa phase) mounting kit announced.
Where To Buy
Sales and support for the MPM System is provided by worldwide partners with experience in neuroscience and advanced research instrumentation.