By clamping as far back from the probe shank as possible, the MPM Probe Mount 1As allows researchers to maximize the density of probes in multi-probe neural recording experiments.  

Mount Maximizes Density of Neuropixels 1.0 Probes in Multi-Probe Recording

New Scale’s MPM-Probe Mount-1As clamps Neuropixels 1.0 probes at the end of the circuit board, where the circuit board attaches to the probe flex cable.

By clamping the probe as far away from the shank as possible, rather than by the silicon spacer or dovetail cap, this probe holder allows researchers to maximize the density of probes in their multi-probe neural recording experiments.

The probe mount design was created in collaboration with the Allen Institute, supporting their development of the six-probe MindScope rig (see photo) and 24-probe next-gen insertion system. Both rigs use New Scale micro manipulators and multi-probe control software.

Photo courtesy Allen Institute  

How to Install and Align Neuropixels 1.0 Probes in the MPM-Probe Mount-1AS

Due to manufacturing variations in the circuit boards of the Neuropixels probes, researchers often need to add shims between the circuit board and probe holder to achieve proper alignment. New Scale’s new probe Alignment Fixture has been demonstrated to be an excellent tool for alignment of both Neuropixels 1.0 and 2.0 probes in their respective probe mounts.

This article describes how to align a Neuropixels 1.0 probe in New Scale’s MPM-Probe Mount-1AS probe holder using the new Alignment Fixture. (For information on Neuropixels 2.0 probes refer to this related article.)

1. Remove the base of the probe holder

Use a screwdriver to loosen the two screws in the MPM-Probe Mount-1AS base. Remove the probe holder from the base.

2. Insert the probe holder in the Alignment Fixture

Loosen the thumbscrew of the Alignment Fixture. Slide the probe holder into the v-groove. With the probe holder in the orientation shown, tighten the thumbscrew.

3. Open the clamp

Use a screwdriver to loosen the screw in the probe holder. Open the probe clamp.

4. Identify the guide features

The probe clamp has a raised edge and a post to aid in probe installation.

5. Install the probe in the holder

Place the slot of the probe flex cable over the post on the probe holder. Place the edge of the probe’s PCB flush against the raised edge.

6. Close the probe clamp

Close the probe clamp and tighten the screw.

7. Check probe alignment

Check the alignment of the probe shank under a microscope, using the reference lines in the Alignment Fixture. The probe holder can be rotated 360° in the Alignment Fixture. Only the two orientations shown below are needed to check for proper alignment.

8. Adjust alignment, using shims if needed

If needed, loosen the probe clamp screw and use shimming material (for example, Kapton Tape ~24 micron thick) between the probe PCB and the Probe Holder to adjust alignment. Re-tighten the probe clamp’s screw and check again.

Photo courtesy Ahmet Arac, UCLA

For more information

Learn about other probe mounts including Neuropixels 2.0 probe mounts

Get probe mount drawings (registration required)

Contact us with questions or to order probe mounts

Note: MPM Probe Mounts are designed for use with the New Scale MPM System. Researchers have successfully adapted them for use with other manipulators. We can’t offer direct support for other manipulators, but will answer questions if we can, and facilitate the open flow of ideas among our users.

Multi-Probe Micromanipulator System

The Alignment Fixture and MPM-Probe Mount 1AS are designed for use with New Scale Technologies’ Multi-Probe Micromanipulator (MPM) system for precise, automated positioning of multiple silicon neural probes.

The compact design enables independent positioning of multiple probes in the smallest space, with ample clear area for a virtual task environment. Automatically and independently move each probe to the optimum location in the brain.